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- Indice
- RISOLUZIONE PROBLEMI
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• Immersion Fluid Tip
•
UV-Activated Chip Sealant Syringe
• UV-Curing Stylus for Chip Sealant
• UV-Curing Stylus Stand
• Isopropanol
• Gloves, powder-free, nitrile
• Heated block (capable of maintaining 40±1°C)
• Scissors
• Cleanroom-grade, low-lint polyester wipes
• Microcentrifuge
• Pipettes and tips, P10 to P1000
• Vortexer
WARNING! ULTRAVIOLET LIGHT HAZARD. Looking directly at a UV light
source can cause serious eye damage. Never look directly at a UV light source
and always prevent others from UV exposure. Follow the manufacturer’s
recommendations for appropriate protective eyewear and clothing.
IMPORTANT! Wear powder-free gloves while preparing the QuantStudio
®
3D
Digital PCR 20K Chips.
1.
Prepare the workspace:
a.
Preheat the heated block to 40°C, the optimal temperature for loading
Digital PCR 20K Chips.
Note: The loading surface should be a flat, temperature-controlled surface
that is large enough to accommodate the Digital PCR 20K Chip consumable.
b.
Allow the prepared digital PCR reaction to equilibrate to room temperature
(approximately 15 minutes).
c.
If necessary, replace the batteries of the UV Stylus in the
UV Stylus Stand.
Note: Replace the batteries of the UV Stylus after 10 hours of use.
2.
Prepare the syringe containing Chip Sealant:
a.
Remove the syringe, plunger, and tip from the protective packaging.
IMPORTANT! Do not discard the brown plastic bag provided. When not in
use, store the Chip Sealant syringe within the bag and in a dark location.
b.
Remove the protective caps from both ends of the syringe, push and twist
the tip to lock it into place, then insert the plunger into the opposite end of
the syringe.
c.
Place the assembled syringe within its package until ready for use.
Load and seal the
Digital PCR 20K
Chips
Chapter 2 Prepare Samples and Load Reactions
Load chips manually
2
QuantStudio
®
3D Digital PCR System User Guide
51
- QuantStudio 1
- 3D Digital PCR System 1
- Contents 3
- (Optional) 5
- About this guide 8
- Prerequisites 9
- Instrument hardware 9
- Instrument software 9
- Instrument consumables 10
- Getting Started 11
- Data collection 12
- Instrument filters 12
- Operating the instrument 13
- Prepare the QuantStudio 14
- Plan the 15
- Choose additional 16
- Install the 17
- Digital PCR 17
- Instrument 17
- IMPORTANT! The QuantStudio 20
- The QuantStudio 21
- Install the thermal 23
- IMPORTANT! The ProFlex 24
- PCR System 26
- CAUTION HOT 27
- Install the GeneAmp 29
- PCR System 9700 29
- IMPORTANT! The GeneAmp 30
- IMPORTANT! If the GeneAmp 31
- Connect the QuantStudio 34
- 3D Instrument to a network 34
- 3D AnalysisSuite 35
- Software 35
- Prepare Samples and Load 36
- Reactions 36
- Sample dilution 37
- Determine the 37
- Prepare the 39
- X0300036 41
- Load and seal the 43
- IMPORTANT! Press the 45
- Load chips manually 50
- Digital PCR 20K 51
- IMPORTANT! Allow the 53
- Perform the PCR 58
- 08:00 AM 4/25/13 25.0°C 60
- GeneAmp® PCR System 9700 60
- Run UserUtilEditCreate 60
- Version: 3.12 60
- Materials 61
- Guidelines for 62
- Unload the 64
- Analyze the Prepared Chips 66
- Analysis workflow 67
- About the 68
- Analyze the chips 69
- Analyzing the data 73
- About the results 74
- About data quality 75
- About the data 75
- Transfer the 76
- Using the QuantStudio 77
- Troubleshooting 79
- (QuantStudio 82
- IMPORTANT! Condensation 83
- Maintenance 86
- Clean the chip tray 87
- Returning your QuantStudio 89
- 3D Instrument for service 89
- Replace the instrument fuses 90
- Replace the fuses 91
- Update the 92
- Calibrate the touchscreen 93
- View the instrument log 93
- Networking 95
- Network setup 96
- Required network 97
- Connect the 98
- Instrument to a 98
- Create a network 103
- Parts and Materials 106
- Specifications and Layout 109
- Instrument clearances 110
- Electrical specifications 111
- Environmental requirements 112
- Network requirements 113
- Symbols on this instrument 114
- Conformity 115
- Location of safety 116
- Fisher Scientific 117
- Instrument safety 117
- 3D Digital PCR Chip Loader 118
- Chemical safety 119
- Biological hazard safety 120
- Documentation and support 121
- 4 February 2015 122
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